Cell Cultured Meat: qRT-PCR

Description

Information regarding qRT-PCR conditions.

Schéma

AttributUnitéLibelléInformationType de donnéesFormat
WellWellIdentifies the specific well on the qPCR plateNumeric
Well_PositionWell_PositionCoordinates of the wellText
Sample_NameSample_NameName or ID of the biological or experimental sampleText
Target_NameTarget_NameTarget GeneText
ReporterReporterFluorescent dye used to detect amplificationText
CTCTThreshold cycle where fluorescence exceeds backgroundNumeric
Ct_MeanCt_MeanAverage CT value from technical replicatesNumeric
Ct_SDCt_SDStandard deviation of CT values among replicatesNumeric
Ct_ThresholdCt_ThresholdFluorescence threshold used to calculate CTNumeric
Baseline_StartBaseline_StartStart cycle for baseline signal calculationNumeric
Baseline_EndBaseline_EndEnd cycle for baseline signal calculationNumeric
Amp_StatusAmp_StatusIndicates whether amplification was detectedText
Cq_ConfCq_ConfConfidence in quantification cycle (Cq) valueNumeric
HIGHSDHIGHSDHigh standard deviation among technical replicates for a given sample, suggesting inconsistent amplificationText
Tm1Tm1Melting temperature of the first PCR product peakNumeric