Cell Cultured Meat: qRT-PCR
Description
Information regarding qRT-PCR conditions.
Schéma
| Attribut | Unité | Libellé | Information | Type de données | Format |
|---|---|---|---|---|---|
| Well | Well | Identifies the specific well on the qPCR plate | Numeric | ||
| Well_Position | Well_Position | Coordinates of the well | Text | ||
| Sample_Name | Sample_Name | Name or ID of the biological or experimental sample | Text | ||
| Target_Name | Target_Name | Target Gene | Text | ||
| Reporter | Reporter | Fluorescent dye used to detect amplification | Text | ||
| CT | CT | Threshold cycle where fluorescence exceeds background | Numeric | ||
| Ct_Mean | Ct_Mean | Average CT value from technical replicates | Numeric | ||
| Ct_SD | Ct_SD | Standard deviation of CT values among replicates | Numeric | ||
| Ct_Threshold | Ct_Threshold | Fluorescence threshold used to calculate CT | Numeric | ||
| Baseline_Start | Baseline_Start | Start cycle for baseline signal calculation | Numeric | ||
| Baseline_End | Baseline_End | End cycle for baseline signal calculation | Numeric | ||
| Amp_Status | Amp_Status | Indicates whether amplification was detected | Text | ||
| Cq_Conf | Cq_Conf | Confidence in quantification cycle (Cq) value | Numeric | ||
| HIGHSD | HIGHSD | High standard deviation among technical replicates for a given sample, suggesting inconsistent amplification | Text | ||
| Tm1 | Tm1 | Melting temperature of the first PCR product peak | Numeric |
